Pure magnesium micro-arc oxidation coating containing nano-SiO2 or nano-TiO2 promotes proliferation and viability of osteoblasts

doi:10.3969/j.issn.2095-4344.2015.03.016

Abstract

Abstract:

BACKGROUND: The micro-arc oxidation technology can enhance the corrosion resistance of magnesium and its alloys to improve the biological properties of the surface.

OBJECTIVE: In order to regulate the biological activity of medical pure magnesium, to modify the micro-arc oxidation coating by adding nano-SiO₂ and nano-TiO₂ into the plating solution, and to study the biological coating effects on osteoblast proliferation and differentiation.

METHODS: Round magnesium sheets were divided into three groups, respectively treated with silicate electrolyte containing 7.5 g/L nano-SiO₂ (nano-SiO₂ group), 4.8 g/L nano-TiO₂ (nano-TiO₂ group), and nothing (control group). Passage 3 osteoblasts were seeded onto the specimen surface in the three groups to observe the early morphology, proliferation and alkaline phosphatase activity of osteoblasts.

RESULTS AND CONCLUSION: Osteoblasts grew well in the nano-SiO₂ group and nano-TiO₂ group, presenting with clear outline, and the cells were long spindle- and polygon-shaped. But in the control group, the cells grew poor in the specimen surface. Cell counting kit-8 test showed that the absorbance values and alkaline phosphatase activities were increased with time in the three groups. Compared with the control group, the proliferative activity was higher in the nano-SiO₂ group and nano-TiO₂ group at days 1, 3, 5 after seeding, while the activity of alkaline phosphatase was also higher in the nano-SiO₂ group and nano-TiO₂ group at days 3, 5 after seeding. These findings indicate the micro-arc oxidation coatings containing nano-SiO₂and nano-TiO₂ can promote osteoblast proliferation and osteogenetic activity, which have good biocompatibility.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: Magnesium, Metal Nanoparticles, Osteoblasts

CLC Number:

R318

Liu Ji-guang, Wang Li-feng, Li Mu-qin, Gao Yan, Wang Xiao-wei . Pure magnesium micro-arc oxidation coating containing nano-SiO2 or nano-TiO2 promotes proliferation and viability of osteoblasts[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(3): 416-420.

Figures/Tables 5

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